Name: Xue, Zheyong
Degree: Ph.D
Title: Professor
Position: Group leader, Academic Leader of Plant Development Subject, Director of Heilongjiang Key Laboratory of Plant Bioactive Substance Biosynthesis and Utilization
Phone number: 86-451-8219-1535
E-mail: zyxue@nefu.edu.cn or xuezhy@126.com
Address: Northeast Forestry University, Shaw (Yifu) Teaching Building, Room 504
Education and Training:
2000 – 2005: Shandong University, Ph.D. in Cell Biology
1994 – 1998: Shandong University, Bachelor of Science in Biochemistry
Employment:
2018 – Now: Northeast Forestry University, Professor
2014 – 2018: Institute of Botany, Chinese Academy of Sciences, Associate Professor
2016 – 2017: Dept. of Metabolic Biology, John Innes Centre, UK, Postdoc
2014 – 2015: Dept. of Metabolic Biology, John Innes Centre, UK, Visiting Scientist
2008 – 2014: Institute of Botany, Chinese Academy of Sciences, Assistant Professor
2005 – 2008: Institute of Botany, Chinese Academy of Sciences, Postdoc
1998 – 2000: Institute of Biotechnology, Ningbo Academy of Agricultural Sciences, Research Associate
Honors and Awards:
n Instructor of The Second Prize et al "Poster Contest of Plant Secondary Metabolism " (2021, 2022);
n Excellent Master's Thesis Instructor of Northeast Forestry University (Miaomiao Liang 2022, Linyong Li 2022);
n Northeast Forestry University Chengdu Science and Technology Award Natural Science Award (2022) third prize, first finisher;
n Laboratory Open Day of Chinese Society of Cell Biology (2022) Laboratory of Excellent Works of Popular Science Activities
Research Specialty:
Metabolic Biology; Synthetic Biology; Plant Molecular Biology; Biochemistry,
Courses to Teach:
Biochemistry, Introduction of Biological Science, Progress in Developmental Biology Frontier, English Writing
Extramural Appointments and Service:
n Director of National Innovation Allance for Plant Natural Active Substance Utilization
n Reserve leader of Heilongjiang provincial leading talent team (2020- present);
n (2022- present);
n Member of National Bioengineering Society; Executive member of Heilongjiang Bioengineering Society; Executive member of Heilongjiang Cell Biology Society;
n Reviewers of National Natural Science Foundation of China (2015- present);
n Letter Evaluation Experts from Degree Center of Ministry of Education of the People's Republic of China;
Editorial Boards:
n Reviewers of Wiley and Elsevier's Publication (2018- present).
n Associate Editorial Board Member of Current Biochemical Engineering (2020-2021);
n Guest Editorial Board of Microbial Cell Factories (2022);
n Leading Guest Editorial Board of the Joint Special Issue of Journal of Cellular Physiology and Molecular Plant Pathology (2022);
Research Interest:
n Metabolic pathway dissection and biosynthesis of plant steroids and triterpenoids
One of my research interests is metabolic pathways of plant on steroids and triterpenoids, and their heterologous biosynthesis using Nicotiana benthamiana etc. plant chassis. Using Omics methodology, we have developed an effective strategy to dissect the biosynthesis pathway for polyphyllins and avenacosides from Paris polyphylla and Avena strigosa. The biosynthesis in plant chassis was established to obtain various active steroids using the multi-genes transient or stable genetic transformation system.
n Metabolic diversity of plant triterpenoids and catalytic mechanism of key enzymes
Oxidosqualene cyclase (OSC) is the key enzyme for the formation of plant triterpenes, and the cyclization process of triterpenes involves many complicated steps. My previous work has characterized several oxidosqualene cyclases (OSC) from rice using the Pichia pastoris expression system. Homologous modeling and site-directed mutation techniques, combined with quantum mechanics-molecular mechanics (QM/MM) multi-scale computational simulation techniques, were used to study the cyclization mechanism of triterpenes, which laid a theoretical foundation for directional design of triterpene synthesis.
Funding:
n Key projects of National Natural Science Foundation of China (Principal investigator of sub-topic), Title: Molecular regulation of the biosynthesis and accumulation of pentacyclic triterpene saponins during in vitro reproduction of Araliaceae forest medicinal plants in Northeast China, 01/01/2022 – 12/23/2025 (U21A20243)
n General project of National Natural Science Foundation of China (Principal investigator), 01/01/2020 – 12/31/2023, Title: Metabolic biosynthetic gene cluster and dissection pathway of oat steroidal saponin (31970314)
n Key project at the central government level (The ability establishment of sustainable use for valuable Chinese medicine resources) (Principal investigator), 01/09/2021– 31/12/2023, Title: Dissection of steroidal saponin metabolic pathway in Paris polyphylla (2060302-2101-17)
Publications: * Corresponding author, **selected publications
Yu Wang#, He Zhang#, Hyol Chol Ri#, Zeyu An#, Xin Wang, Jianan Zhou, Dongran Zheng, Hao Wu, Pengchao Wang, Jianfei Yang, Dingkun Liu, Diyang Zhang, Wenchieh Tsai, Zheyong Xue, Zhichao Xu, Peng Zhang*, Zhongjian Liu*, Hailong Shen*, Yuhua Li*. (2022) Deletion and tandem duplications of biosynthetic genes drive the diversity of triterpenoids in Aralia elata. Nature Communications 13: 3224
https://doi.org/10.1038/s41467-022-29908-y
He Zhang#, Xin Hua#, Dongran Zheng, Hao Wu, Chuanwang Li, Pan Rao, Mengliang Wen, Yong Eui Choi, Zheyong Xue*, Yu Wang*, Yuhua Li *. (2022) De novo biosynthesis of oleanane-type ginsenosides in Saccharomyces cerevisiae realized by characterizing and engineering two distinguished types of glycosyltransferases from Panax ginseng. Journal of Agricultural and Food Chemistry 70(7): 2231–2240
https://doi.org/10.1021/acs.jafc.1c07526
Miaomiao Liang#, Xueliang Ge#, Hui Xua, Kaifeng Ma, Wei Zhang, Yibo Zan, Thomas Efferth, Zheyong Xue*, Xin Hua*. (2022) Phytochemicals with activity against methicillin-resistant Staphylococcus aureus. Phytomedicine 100:154073
https://doi.org/10.1016/j.phymed.2022.154073
Wei Song#, Chunchun Zhang#, Jiali Wu, Jianzhao Qi, Xin Hua, Liping Kang*, Qiang Yuan*, Jifeng Yuan*, Zheyong Xue*. (2022) Characterization of three Paris polyphylla glycosyltransferases from different UGT families for steroid functionalization. ACS Synthetic Biology 11(4): 1669-1680**
https://doi.org/10.1021/acssynbio.2c00103
Miaomiao Liang#, Zhang Fan#, Jiaxin Xu, Xiaoning Wang, Wu Ruibo*, Zheyong Xue*. (2022) A conserved mechanism affecting hydride shifting and deprotonation in the synthesis of hopane-type triterpenes as the wax compositions in oat. Proc Nat Acad Sci U.S.A. 119：e2118709119**
https://www.pnas.org/doi/abs/10.1073/pnas.2118709119
Xin Hua#, Wei Song#, Kangzong Wang#, Xue Yin, Changqi Hao, Zhichao Xu*, Tongbing Su*, Zheyong Xue*. (2022) Effective prediction of biosynthetic pathway genes involved in bioactive polyphyllins in Paris polyphylla. Communications Biology 5:50**
https://doi.org/10.1038/s42003-022-03000-z
Jing Wang, Yanhong Guo, Xue Yin, Xiaoning Wang*, Xiaoquan Qi*, Zheyong Xue*. (2022) Diverse triterpene skeletons are derived from the expansion and divergent evolution of 2,3-oxidosqualene cyclases in plants. Crit Rev Biochem Mol. 57(2): 113-132**
https://doi.org/10.1080/10409238.2021.1979458
Changqi Hao, Shuairun Wang, Yi Wang, Xinyi Hou, Yaxuan Jiang, Bowen Jiang, Xiuli Zhang, Xin Hua and Zheyong Xue*. (2021) Technology and application of hairy root culture in monocotyledons. Current Biochemical Engineering 7(1) 31-37.
https://doi.org/10.2174/2212711907666210525115018
Yan Li#, Aymeric Leveau#, Qiang Zhao#, Qi Feng, Hengyun Lu, Jiashun Miao, Zheyong Xue, …Bin Han*, Anne Osbourn*. (2021) Subtelomeric assembly of a multi-gene pathway for antimicrobial defense compounds in cereals. Nature Communications 12:2563
https://doi.org/10.1038/s41467-021-22920-8
Haizheng Yu, Dongyue Li, Dongfeng Yang, Zheyong Xue, Jie Li, Bingcong Xing, Kaijing Yan, Ruilian Han*, Zongsuo Liang*. (2021) SmKFB5 Protein regulate phenolic acids biosynthesis via controlling the degradation of phenylalanine ammonia-lyase in Salvia miltiorrhiza. Journal of Experimental Botany 72(13):4915-4929.
https://doi.org/10.1093/jxb/erab172
Xufan Lian#, Xiuli Zhang#, Fei Wang, Xiaoning Wang, Zheyong Xue*, Xiaoquan Qi*. (2020) Characterization of a 2,3-oxidosqualene cyclase in the toosendanin biosynthetic pathway of Melia toosendan. Physiologia Plantarum 170(4):528-536.
https://doi:10.1111/ppl.13189
Shengnan Tan, Xin Hua*, Zheyong Xue* and Jianzhang Ma*. (2020) Cajanin stilbene acid inhibited vancomycin-resistant enterococcus by inhibiting phosphotransferase system. Frontiers in Pharmacology 11:473.
https://doi.org/10.3389/fphar.2020.00473
Linggai Cao#，Hao Wu#, He Zhang, Quan Zhao, Xue Yin, Dongran Zheng, Chuanwang Li, MinJun Kim, Pyol Kim, Zheyong Xue*, Yu Wang*, Yuhua Li*. (2020) Highly efficient production of diverse rare ginsenosides using combinatorial biotechnology. Biotechnology and Bioengineering 117(6)：1615-1627**
https://doi.org/10.1002/bit.27325
2018
Zheyong Xue#, Zhengwei Tan#, Ancheng Huang, Yuan Zhou, Juncong Sun, Xiaoning Wang, Ramesha B. Thimmappa, Michael J. Stephenson, Anne Osbourn*, Xiaoquan Qi*. (2018) Identification of key amino acid residues determining product specificity of 2, 3-oxdiosqualene cyclase in Oryza species. New Phytologist 218(3):1076-1088**.
https://doi.org/10.1111/nph.15080
Zheyong Xue#, Xia Xu#, Yuan Zhou#, Xiaoning Wang, Yingchun Zhang, Dan Liu, Binbin Zhao, Lixin Duan, Xiaoquan Qi*. (2018) Deficiency of a triterpene pathway results in humidity-sensitive genic male sterility in rice. Nature Communications 9(1): 604**.
https://doi:10.1038/s41467-018-03048-8
Yanling Liu#, Zhongjuan Zhao#, Zheyong Xue#, Peng Wang#, Yunfei Cai, Tiandi Wei, Jing Gong and Fengning Xiang*. (2016) An intron less beta-amyrin synthase gene is more efficient in oleanolic acid accumulation than its paralog in Gentiana straminea. Scientific Reports 6: 33364 **.
https://xs.scihub.ltd/https://doi.org/10.1038/srep33364
Shuo Li1, Nan Wang1, Dandan Ji1, Zheyong Xue, Haiyan Guo, Yupei Jiang and Fengning Xiang*. (2016) Evolutionary and functional analysis of membrane-bound NAC transcription factor genes in soybean. Plant Physiology 172(3):1804-1820.
https://doi.org/10.1104/pp.16.01132
Guanghong Cui, Lixin Duan, Baolong Jin, Jun Qian, Zheyong Xue, Guoan Shen, John Hugh Snyder, Jingyuan Song, Shilin Chen, Luqi Huang, Reuben J Peters, Xiaoquan Qi*. (2015) Functional divergence of diterpene syntheses in the medicinal plant Salvia miltiorrhiza bunge. Plant Physiology 169(3): 1607-1618.
https://doi.org/10.1104/pp.15.00695
Xu Chi, Yingchun Zhang, Zheyong Xue, Laibao Feng, Huaqin Liu, Feng Wang, Xiaoquan Qi*. (2014) Discovery of rare mutations in extensively pooled DNA samples using multiple target enrichment. Plant Biotechnology Journal 12(6):709–717.
https://doi.org/10.1111/pbi.12174
Juncong Sun#, Xia Xu#, Zheyong Xue#, John Hugh Snyder, Xiaoquan Qi*. (2013) Functional analysis of a rice oxidosqualene cyclase through total gene synthesis. Molecular Plant 6(5): 1726-1729**.
https://doi.org/10.1093/mp/sst038
Zheyong Xue, Lixin Duan, Dan Liu, Jie Guo, Song Ge, Jo Dicks, Paul O ´ Ma´ille, Anne Osbourn, Xiaoquan Qi*. (2012) Divergent evolution of oxidosqualene cyclases in plants. New Phytologist 193(4):1022-1038**.
https://doi.org/10.1111/j.1469-8137.2011.03997.x
