Name: Liu, Chengwei
Degree: Ph.D
Title: Professor
Position: Professor
Phone number:
E-mail: liuchw@nefu.edu.cn
Address: Northeast Forestry University, Shaw (Yifu) Teaching Building, Room 617
Education and Training:
2014/04 – 2015/10: Hokkaido University, Postdoctoral Training in chemistry
2011/04 – 2014/03: Hokkaido University, Ph.D. in Chemical Sciences
2009/04 – 2011/03: Akita Prefectural University, Master in Bioresource
2008/03 – 2009/03: Akita Prefectural University, Research student
2004/07: Wuhan Bioengineering Institute
Employment:
2019/11 – : Northeast Forestry University, Professor
2015/11 – 2019/09: Hokkaido University, Assistant Professor
2004/08 – 2008/02: HEC pharm group, Researcher
Research Specialty:
Synthetic Biology, Natural Product Chemistry, Biosynthetic Pathway.
Research Interest:
My research is focused on natural products from fungi, mushrooms, plants, and many other natural products that are used as medicines. In recent years, with the development of the genome project, the genome sequence becomes easy to utilize. Based on genomic information, many secondary metabolite biosynthesis genes are suppressed. We are aimed to activate potential secondary metabolite gene clusters to produce new natural products.
We are using the CRISPR/Cas9 approach for genome editing and Aspergillus oryzae as a heterologous expression host for the synthesis of natural products. Using this method, we will be able to elucidate unexplored natural product biosynthesis machinery and produce new compounds. In the future, we will synthesize natural product analogues using organically synthesized substrates. Eventually, we expect to use related enzymes to produce new materials that are not present in the original biosynthetic pathways.
Funding:
(1) The Japan Society for the Promotion of Science (JSPS)
04/01/2018 – 03/31/2020,
Title: Development of heterogeneous production method for mushroom terpene compounds
(2) The Japan Society for the Promotion of Science (JSPS)
04/01/2016 – 03/31/2018
Title: Elucidation and controlment of diverse structure in the biosynthesis of indole diterpene
Publications:
1. Chengwei Liu, Atsushi Minami, Taro Ozaki, Jing Wu, Hirokazu Kawagishi, Jun-ichi Maruyama, Hideaki Oikawa*.
Efficient reconstitution of Basidiomycota diterpene erinacine gene cluster in Ascomycota host Aspergillus oryzae based on genomic DNA sequences.
J. Am. Chem. Soc., 141 (39), 15519-15523 2019. doi: 10.1021/jacs.9b08935.
2. Chengwei Liu, Atsushi Minami, Tohru Dairi, Katsuya Gomi, Barry Scott, Hideaki Oikawa*.
Biosynthesis of Shearinine: Diversification of a Tandem Prenyl Moiety of Fungal Indole Diterpenes.
Org. Lett. 18 (19), 5026-5029, 2016. DOI: 10.1021/acs.orglett.6b02482.
3. Chengwei Liu, Koichi Tagami, Atsushi Minami, Tomoyuki Matsumoto, Jens Christian Frisvad, Hideyuki Suuki, Jun Ishikawa, Katsuya Gomi, Hideaki Oikawa*.
Reconstitution of biosynthetic machinery for high elaborated indole diterpene penitrem.
Angew. Chem. Int. Ed. Engl. 54 (19), 5748-52, 2015. DOI: 10.1002/anie.201501072. (Hot Paper, Cover Picture)
4. Chengwei Liu, Motoyoshi Noike, Atsushi Minami, Hideaki Oikawa, Tohru Dairi*. A fungal prenyltransferase catalyzes the regular di-prenylation at positions 20 and 21 of paxilline. Biosci. Biotechnol. Biochem. 78 (4), 448-54, 2014. doi:10.1080/09168451.2014.882759.
5. Chengwei Liu, Motoyoshi Noike, Atsushi Minami, Hideaki Oikawa, Tohru Dairi*. Functional analysis of a prenyltransferase gene (paxD) in the paxilline biosynthetic gene cluster. Appl. Microbiol. Biotechnol. 98 (1), 199-06, 2014. DOI: 10.1007/s00253-013-4834-9.
6. Chengwei Liu, Atsushi Minami, Motoyoshi Noike, Hiroaki Toshima, Hideaki Oikawa, Tohru Dairi*. Regiospecificities and prenylation mode specificities of the fungal indole diterpene prenyltransferases AtmD and PaxD. Appl. Environ. Microbiol. 79 (23), 7298-304, 2013. doi: 10.1128/AEM.02496-13.
7. Chengwei Liu, Tatsuya Fukumoto, Tadashi Matsumoto, Patrizia Gena, Daniele Frascaria, Tomoyuki Kaneko, Maki Katsuhara, Shihua Zhong, Xiaoli Sun, Yanming Zhu, Ikuko Iwasaki, Xiaodong Ding, Giuseppe Calamita, Yoshichika Kitagawa*. Aquaporin OsPIP1;1 promotes rice salt resistance and seed germination. Plant. Physiol. Biochem. 63, 151-158, 2013. doi: 10.1016/j.plaphy.2012.11.018.
