Name: Lan, Xingguo

Degree: Ph.D.

Title: Associate Professor

Phone number: 086-13633637286

E-mail: lanxingguo@126.com

Address: Northeast Forestry University, Shaw (Yifu) Teaching Building, Room 226

Education and Training:

1998.09 – 2002.07: Northeast Forestry University, Bachelor of Science in Biology

2002.09 – 2007.07: Northeast Forestry University, Ph.D. in Developmental Biology

Employment:

2007.07 – 2010.09: Northeast Forestry University, Lecturer

2010.09 – Present: Northeast Forestry University, Associate Professor

Research Specialty:

Molecular Biology, Proteomics, Plant Development, Plant Reproduction

Courses to Teach:

Molecular Biology, Proteomics

Research Interest:

My research interest is self-incompatibility (SI) in Brassica. To avoid inbreeding depression and promote out-crossing, many plants have adopted self-incompatibility systems, which enable the plant to discriminate between ‘‘self’’ and ‘‘non-self’’ pollen, to maintain genetic diversity in the population. This discrimination of self and non-self between pollen and pistil is followed by selective inhibition of self-pollen germination and/or growth. I am particularly interested in utilizing the Brassica SI system to understand the molecular mechanisms behind pollen-pistil interactions. My research is to identify other components involved in the SI response in Brassica using the yeast two hybrid system for ARC1-interacting proteins, construct over-expression, RNAi-expression constructs and CRISPR-Cas9 constructs of targeting gene using binary vector that will be used for transforming Brassica; isolate the relevant genes and proteins involved in stigma development and characterize their biochemical activity.

Funding:

(1) National Natural Science Foundation of China (31870300) (P.I.)

01/01/2019 – 12/31/2022,

Title: Functional analysis of heat shock protein JDP1 in self-incompatibility in Brassica napus

(2) Fundamental Research Funds for the Central Universities, China (2572018BD01) (P.I.)

05/01/2018 – 04/30/2021

Title: Investigation of ARC1-mediate pathway in Brassica Self-incompatibility

(3) National Natural Science Foundation of China (30900115) (P.I.)

01/01/2010 – 12/31/2012,

Title: Screening and functional analysis of ARC1-interacting proteins from ornamental kale (Brassica oleracea var. acephala)

(4) Fundamental Research Funds for the Central Universities, China (DL13CA13) (P.I.)

04/01/2013 – 03/31/2017

Title: Comparative proteomic analysis of developmental stage of stigma from ornamental kale (Brassica oleracea var. acephala)

(5) Fundamental Research Funds for the Central Universities, China (DL09BA08) (P.I.)

01/01/2010 – 12/31/2012

Title: Cloning and functional analysis of ARC1 promoter of ornamental kale (Brassica oleracea var. acephala)

Publications:

1）Li X, Kumar A, Zhang T, Wei D, Li Y, Lan X*, Samuel MA. Temporal regulation of two cytosolic phosphoglucomutases during stigma development in ornamental kale (Brassica oleracea var. acephala). Plant Signal Behav. 2018;13(5): e1467698. DOI: 10.1080/15592324. 2018.1467698

2）Lan X, Yang J, Kumar A, Nie Y, Li X, Li Y, Samuel MA. Flavonoids and ROS play opposing roles in mediating pollination in ornamental kale (Brassica oleracea var. acephala). Mol Plant. 2017, 10: 1361-1364. DOI: 10.1016/j.molp.2017.08.002

3）Lan X, Yang J, Cao M, Wang Y, Kawabata S, Li Y. Isolation and characterization of a J domain protein that interacts with ARC1 from ornamental kale (Brassica oleracea var. acephala). Plant Cell Rep. 2015, 34: 817-829. DOI: 10.1007/s00299-015-1744-6

007/s00299-015-1744-6