Name:Xu, Zhiru

Degree: Ph.D.

Title: Associate Professor

Phone number: 86-451-82191783

E-mail: xuzhiru2003@nefu.edu.cn

Address: Northeast Forestry University, Shaw (Yifu) Teaching Building, Room 224

Education and Training:

1989 – 1993: Harbin Normal University, Bachelor of Biology Education

1993 – 1996: Harbin Normal University, Master of Botany

2001 – 2005: Northeast Forestry University, Ph.D. in Tree Genetics and Breeding

2005 – 2007: Northeast Forestry University, Postdoctoral Training in Biology

Employment:

1996 – 1999: Northeast Forestry University, Research Associate

1999 – 2005: Northeast Forestry University, Lecturer

2005 – present: Northeast Forestry University, Associate Professor

Courses to Teach：

Molecular Biology; Biochemistry; Biochemistry Experiment; Newest Theory and Technique of Molecular Biology

Research Specialty:

Plant Molecular Biology,Plant Resistance Biology, Forest Resistance Breeding,Gene Transduction, Microbiology.

Research Interest:

My research work is involved in the light-dependent and light-independent anthocyanidin biosynthesis and its regulatory mechanism, as well as forest resistance mechanism (copper molecular chaperone). The biosynthesis of anthocyanin in flowers and fruits has different correlation with light. Some ones are often intensity influenced by light, while it does not require light irradiation for anthocyanin biosynthesis in others. Some important genes which encoded the major catalyzed enzymes of anthocyanin biosynthesis were cloned from ‘Tsuda’ and ‘Akamaru’ turnips. We identified the expression trait of these genes. The over-expression and RNAi vector of thesegenes were constructed and the transgenic tobacco plants were obtained. We cloned the promoter sequences ofthesegenes and identified the bioactivity of the whole promoter and the different elements by GUS histochemical staining. These results will provide important study data for further clarifing the mechanism of light-independent and independen anthocyanin biosynthesis.

Otherwise, Atx1-like copper chaperones in yeast and animals have been studied extensively. There are two types of Atx1-like copper chaperones in plants, such as Atx1-type and CCH-type. CCH-type copper chaperone has the plant exclusive C-terminal extension. At present, the study on this kind of copper chaperone was lagged relatively. We used Populus simonii x P. nigra as material to study the expression feature of CCH copper chaperones family genes. Using the high throughput sequencing method, we would to compare the differentially expressed genes between overexpression transgenic plants and the inhibited expression ones. Currently, the function of motifs in the promoter of CCH family genes was ascertaining. Then, we could screen the regulatory factors interacting with the cis-acting elements of CCH genes in poplar. The interacting protein of CCH copper chaperones family members would be filtered and the role of the C-terminal in these interactions could be confirmed.

Funding:

(1) National Natural Science Foundation of China (31470664) (P.I.), 01/01/2015-12/31/2018,

Title: Analysis of related metabolic pathways involved in copper stress participated by CCH copper chaperone protein family in Populus

(2) National Natural Science Foundation of China (30700560) (P.I.), 01/01/2008-12/31/2010,

Title: Functional study and light induction expression trait of anthocyanin biosynthesis geneDFR

Publications:

1. Qu C P,Xu Z R, Hu Y B, Lu Y, Yang C J, Sun G Y, Liu G J. RNA-SEQ reveals transcriptional level changes of poplar roots in different forms of nitrogen treatments. Front Plant Sci, 2016, 7:51, doi: 10.3389/fpls.2016.00051(SCI)

2. Wu X Y, Yang H, Qu C P,Xu Z R, Li W, Hao B Q, Yang C P, Sun G Y, Liu G J. Sequence and expression analysis of theAMTgene family in poplar. Front Plant Sci, 2015,6:337, doi: 10.3389/fpls.2015.00337(SCI)

3. Zheng M Z, Qu C P, Liu G J, Wei Z G, Xu Z R. Differential expression of cysteine protease inhibitor (CPI) gene of Polygonum sibiricum Laxm. leaves, stems and rhizomes in response to NaHCO3. AFR J Biotechnol, 2012, 11(20): 4484-4492 (SCI)

4. Wang Y, Liu G J, Yan X F, Wei Z G,Xu Z R. MeJA-inducible expression of the heterologous JAZ2 promoter from Arabidopsis in Populus trichocarpa protoplasts. J Plant Dis Protect, 2011, 118(2): 69-74(SCI)

5. Qu C P, Xu Z R, Liu G J, Liu C, Li Y, Wei Z G, Liu G F. Differential expression of copper-zinc superoxide dismutase gene of Polygonum sibiricum leaves, stems and underground stems, subjected to high-salt stress. Int J Mol Sci, 2010, 11(12): 5234-5245（SCI）

6.Xu Z R, Hou J, Tong L, Cui G X, Liu G J, Li Y H. Cloning and expression trait of UDP- glucose: flavonoid 3-O-glucosyltransferase gene (UF3GT) in turnip. AFR J Biotechnol, 2012, 11(71): 13540-13545

7. Ma J, Qu C P,Xu Z R, Yang C P, Liu G J. Salt tolerance of transgenic tobacco containingPsATXandPsSODgenes ofPloygonum sibiricumLaxm.. Bulletin of Botanical Research,2015, 35(2): 208-217

8.Xu Z R, Ma J, Cui G X, Liu T, Liu G J. Functional identification and promoter preliminary analysis of flavonoid 3’-hydroxylase genes in turnip. Bulletin of Botanical Research,2015, 35(4): 572-582

9.Xu Z R, Liu T, Cui G X, Li C L, Ma J, Li Y H. Cloning and function identification of dihydroflavonol 4-reductase genes in turnip. Acta Horticulturae Sinica,2014, 41(4): 687-700

10.Xu Z R, Ma J, Cui G X, Li Y H. Cloning and functional analysis of the key enzyme DFR promoters in turnip anthocyanin biosynthesis. Acta Horticulturae Sinica,2014, 41(8): 1631-1641

11.Xu Z R, Ma J, Hou J, Tong L, Li Y H. Comparison on the regeneration system and genetic transformation condition in 4 ground cover chrysanthemum cultivars. Chinese Agricultural Science Bulletin,2014, 30(7): 130-137

12.Xu Z R, Chen Z H, Jiang Y D, Hou J, Tong L, Li Y H. Establishment of the regeneration system and genetic transformation ofBrDFRgene in chrysanthemum cultivar. Acta Horticulturae Sinica,2013, 40(8): 1517-1526

Books:

1. Xu Z R, Na D C, Li Y F, Zheng G X. Research technique and method of Environment Molecular Biology. Harbin Institute of Technology Press, 2012

2.Xu Z R, Zhou B, Lan X G. Problem Set of Molecular Biology. Northeast Forestry University Press, 2010

3. Li Y H, Liu J H, Xu Q J,Xu Z R. Handbook of Modern Molecular Biology Module Experiment. Higher Education Press, 2007

Patents:

1.Xu Z R, Wang B C, Liu G J, Liu C C, Liu M K, Wei Z G, Liu G F, Yang C P. 2011. Method of constructing a two valence expressing plant vector pROKⅡ and the universal primer, Assigned by State Intellectual Property Office of the People's Republic of China, Patent No.: ZL 2008 1 0064790. 4

2. Liu G J, Liu G F, Yang C P, Liu M K, Yan X F,Xu Z R, Wei Z G, Wang B C. 2010. A way to build a yeast two-value general-purpose expression vector. Assigned by State Intellectual Property Office of the People's Republic ofChina, Patent No.: ZL 2008 1 0209569. 3