Name: Wang, Chunsheng
Degree: Ph.D.
Title: Associate Professor
Position: Dean of the laboratory of animal embryos
Phone number: 13946012096
E-mail: wangchunsheng79@nefu.edu.cn
Address: Northeast Forestry University, Yifu teaching building, Room 136
Education and Training:
1997 – 2001 B.S. in Animal Science,Department of Animal Science & Technology, Northeast Agricultural University, Harbin, China;
2001 –2005 M.S. in Animal Genetic Breeding and Reproduction,college of Animal Science & Technology, Northeast Agricultural University, Harbin, China;
2005 – 2008 Ph.D. in Animal Genetic Breeding and Reproduction,college of Animal Science & Technology, Northeast Agricultural University, Harbin, China;
2008 – 2014: Northeast Forestry University, Postdoctoral Training in Development Biology
Employment:
2005 – 2008 Assistant Professor，College of Life Science , NEFU, China
2008 – 2012 Lecturer，College of Life Science, NEFU, China
2012 – Present Associate Professor，College of Life Science , NEFU, China
Honors and Awards:
2014: Third Place in Teaching Competition of NEFU
Research Specialty: Animal somatic cell reprogramming and transgenic technology
Courses to Teach: Animal anatomy and histology; Developmental biology
Extramural Appointments and Service:
Executive director, Association of Rabbitry in Animal husbandry association of Heilongjiang Province
Research Interest:
A major focus of my work is1) Exploration and Utilization of Livestock Stem Cells;2) Molecular mechanism of fur animal virus infection in target cells
Funding:
(1) the Fundamental Research Funds for the Central Universities[DL10BA06] (P.I.)
2010.10-2013.10
Title: Study on the induction of pluripotent stem cells from sheep somatic cells
(2) National Natural Science Foundation of China[31000990] (P.I.)
2011.1.1-2013.12.31Title: Induction of Pluripotent Stem Cells from Sheep Fibroblasts by Heterogenous Factors and its Methylation of Pluripotent Gene Promoters
(3) the Fundamental Research Funds for the Central Universities[DL13EA06-02] (P.I.)
2013.4-2016.3
Title: The role and molecular mechanism of muscle development regulatory factors in the differentiation of iPS into muscle cells
(4) the Fundamental Research Funds for the Central Universities[2572016CA10] (P.I.)
2016.3-2019.2
Title: establishment of tissue specific gene knockout system based on the CRISPR/Cas9
(5) National Natural Science Foundation of Heilongjiang province[C2016012] (P.I.)
2016.7-2019.6
Title: inducing sheep umbilical cord mesenchymal stem cells into hair follicle cells and Wnt pathways in the role of the process
(6) Collaborative research project(P.I.)
Optimization of sheep mesenchymal stem cell culture system.
2021-2023.
(7) Collaborative research project(P.I.)
Optimization of large-scale culture process for sheep umbilical cord mesenchymal stem cells.
2022-2023.
Publications:
1.Chunsheng Wang, Fang Luo, Tengzi Zhang,Jianling Zhao, Shanhua Piao, Tiezhu An. Purification and Preparation of Anti-serum to Spider Dragline Silk Protein. Sichuan Journal of Zoology, 2011.30(4): 506-509.
2.Chunsheng Wang, Yuefei Yang, Fanyong Ning, Chao Xue, Shanhua Piao, Tiezhu An. Construction and Detection of Retrovirus Vector with Sheep Klf4 Gene. Chinese Journal of Veterinary Science, 2011.31(8): 1118-1122.
3.Chunsheng Wang, Lu Yuan, Fangyong Ning, Zhihao Wu, Shanhua Piao, Tiezhu An. Filtration of Transgenic Sheep Skin Fibroblasts with KAP6.1-GFP-polymerized Spider Dragline Silk Protein Gene(4S). Scientia Agricultura Sinica, 2011.44(12):2561-2566.
4.Chunsheng Wang, Jing Li, Fangyong Ning, Qiuting Zhang, Yang Liang, Shanhua Piao, Tiezhu An. Idengtification of Transgenic Mouse Fibroblasts with UHS-Polymerized Spider Dragline Silk Protein Gene(2S). Acta Agriculture Universitatis Jiangxiensis, 2011.33(6):1202-1205.
5.Chunsheng Wang,Fangyong Ning, Hao Li, Qiuting Zhang, Shanhua Piao, Tiezhu An. The Influence of Multi-mating on Female Reproduction of Jin Zhou Black Standard Mink. Journal of Economic Animal, 2011.15(1):6-9.
6.Xinglan An, Chao Xue, Shanhua Piao, Xiangyang Miao, Chunbo Teng, Tiezhu An, Chunsheng Wang*. Construction of RNAi Vectors of Mouse Myostatin Gene and the Detection of its RNAi Efficiency. Heilongjiang Animal Science and Veterinary Medicine, 2012.4:44-47.
7.Jianling Zhao, Tiezhu An, Zhiren Zhang, Zizhu Wang, Shanhua Piao, Chunsheng Wang*. Construction and Detection of Retrovirus Vector with Ovine Sox2 Gene. Chinese Animal Husbandry and Veterinary Medicine, 2012.39(5):217-220.
8.Chunsheng Wang, Zhiren Zhang, Shanhua Piao, Tiezhu An. Role of MicroRNA in Induced Pluripotent Stem Cell. Hereditas, 2012.34(12):1545-1550.
9.Zizhu Wang, Tiezhu An, Hao Li, Shanhua Piao, Chunsheng Wang*. Cloning and Coding Sequence Analysis of Sheep c-Myc Gene. Grass-Feedng Livestock, 2013.1:24-28.
10.Zhiren Zhang, Tiezhu An, Zizhu Wang, Shanhua Piao, Chunsheng Wang*. Expression of miR-193b and its Target Gene Kit in Different Colors of Alpaca Skin. Chinese Animal Husbandry and Veterinary Medicine, 2013.40(5):49-53.
11.Chenrui An, Tiezhu An, Hao Li, Shanhua Piao, Chunsheng Wang*. Cloning and Sequence Analysis of c-Myc Gene in Goat. Chinese Animal Husbandry and Veterinary Medicine, 2014.41(4):154-159.
12.Hongwei Song, Tiezhu An, Shanhua Piao, Chunsheng Wang*. Mammalian DNA Methylation and its Roles during the Induced Reprogramming of Somatic Cells. Hereditas, 2014.36(5):431-438.
13.Chunsheng Wang, Yongle Li, Yuge Qiu, Shanhua Piao, Tiezhu An. The Screening and Identification of Transgenic Goat Fibroblast Cells with Sweet Protein Brazzein Gene. Journal of Anhui Agricultural Science, 2014.42(24):8099-8101.
14.Chunsheng Wang, Zhichong Zhang, Qiuting Zhang, Shanhua Piao, Yuge Qiu, Tiezhu An. The Screening and Identification of Transgenic Goat Mammary Epithelial Cells with Temporin Gene. Acta Agriculturae Universitatis Jiangxiensis (Natural Sciences Edition), 2014.36(3):609-613.
15.Weiwen Song, Tiezhu An, Chunsheng Wang*. microRNAs Play Roles in Differentiation of Pluripotent Stem Cells into Myocardial Cells. Chinese Journal of Biochemistry and Molecular Biology, 2015.31(3):244-250.
16.Qi Zhao, Zheng Guo, Shanhua Piao, Chunsheng Wang*. Discovery of Porcine Maternal Factors Related to Nuclear Reprogramming and Early Embryo Development by Proteomic Analysis. Proteome Science, (2015) 13:18.
DOI 10.1186/s12953-015-0074-5
17.Hongyao Sun, Kun Hou, Weizhi Wang, Cuicui Zhou, Tiezhu An, Chunsheng Wang*. Cloning and Sequence Analysis of Lin28 Gene Coding Region from Sheep. Chinese Animal Husbandry and Veterinary Medicine, 2015.42(10):2567-2572.
18. Hao Li，Jie Zhang，S.H.Piao，S.H.Piao，Chunsheng Wang*.Expression and activity analysis of sheep Oct -4 gene promoter.Heilongjiang Animal Science and Veterinary Medicine, 2016,19:1-4.
19.Q.Zhao,Y.G.Qiu,J.T.Tian,C.S.Wang*& T.Z.An*.Porcine Cloned Embryos Reconstructed with the Cell Nuclei of Tetraploid M- phase Fibroblast Cells Can Restore Normal Diploidy at the Blastocyst Stage.Anim Biotechnol. 2017 Jul 3;28(3):182-188. doi: 10.1080/10495398.2016.1249794.
20.Hu Guo，Xucheng Zhang,Xuzhu Huyun,Meiqi Shang,T.Z.An, Chunsheng Wang*.Molecular clonibg and expression activity detection of sheep Nanog promoter.Chinese Journal of Veterinary Science，2017, 37 (7) :1316-1321.
21. Qi zhao，Hu Guo，Guofeng Fu，Jianxing Lian，Mengjie Guo，T.Z.An，Chunsheng Wang*. Screening of Transgenic SNanog-GFP Sheep Fibroblast Cell Line.Heilongjiang Animal Science and Veterinary Medicine, 2017,9:130-133.
22. Bao Zhang,S.H.Piao，Chunsheng Wang*.Research progress on menthods of delivering reprogramming factor into cells in the process of induced pluripotent stem cell. Heilongjiang Animal Science and Veterinary Medicine, 2018, 19 : 36-40.
23. Wenjing Du,Hao Li，Chao Xue，S.H.Piao，Chunsheng Wang*，T.Z.An*.Transpiantation and identification of reconstructed embryos with MSTN gene knockdown from s.Journal of Hunan Agricultural University，2018,44（5）：539-543.
24. Wenjing Du，Weiwen Song，Shengnan Chen，Shanhua Piao，Chunsheng Wang*，T.Z.An*.Study on the differentiation of sheep umbilical cord mesenchymal stem cells into myoblasts induced by microRNA-133.Heilongjiang Animal Science and Veterinary Medicine，2018(23): 10-12，264.
25. Wenjing Du，Yang Liang，S.H.Piao，Chunsheng Wang*,T.Z.An*.Preparation of retrovirus－mediated spider dragline silk gene transgenic mice.Heilongjiang Animal Science and Veterinary Medicine，2018(17):10-12，230.
26. Xinyi Gao，Cong Wang，Jingting Wu，S.H.Piao，T.Z.An，Chunsheng Wang*.Cloning and sequence analysis of MC1R gene CDS region in Neovisonvison.Heilongjiang Animal Science and Veterinary Medicine，2019,11:174-150.
27. Hao Li,Shengnan Chen，Songmei li，S.H.Piao，T.Z.An，Chunsheng Wang*.Generation of muscle-specific gene-targeted mouse embryos expressing Cas9 protein.Acta Lab Anim Sci Sin，2019,27(3) : 271-277.
28. Li Tian，Shaohui Shi，Hongyu Hu，Shengnan Chen，Songmei li，Chunsheng Wang*.Sequence Analysis and Cloning of Oct4 Gene in Sheep. Grass-Feeding Livestock,2019,3:19-24.
29. Shengnan Chen，Hongwei Song,Songmei Li,S.H.Piao，Chunsheng Wang*.Biological characteristics and prokaryotic expression of the rpoN gene of Aeromonas veronii TH0426 Strain[J].Heilongjiang Animal Science and Veterinary Medicine,2020(01):22-26.
30.Li, S.N. Chen, S.H.Piao, T.Z.An, C.S.Wang*Production of Artificial Synthetic Spidroin Gene 4S-transgenic Cloned Sheep Embryos using Somatic Cell Nuclear Transfer.Anim Biotechnol.2020,(14):1-11. DOI: 10.1080/10495398.2020.1737098
31. Yifan lv，Gengdong Li，Nan Xue，Guoliang Lv，Shaohui Shi，Chunsheng Wang*.Prokaryotic Expression，Purification of LbCpf1 Protein Gene and in Vitro Cleavage Activity Assay[J].China Biotechnology，2020,40（8）：59-63.
32. Duoling Sun，Heng Zhao，Hanqing Feng，Shengnan Chen，Songmei Li，Chunsheng Wang*.Construction and expression detection of Pax7 gene lentiviral vector in sheep[J].Heilongjiang Animal Science and Veterinary Medicine,2020(19):11-15,165.
33. Songmei Li，Yuge Qiu，Shengnan Chen，Xiaomeng Wang，Chunsheng Wang*.CRISPR/Cas9 Mediated Exogenous Gene Knock-in at ROSA26 Locus in Sheep Umbilical Cord Mesenchymal Stem Cells[J].Scientia Agricultura Sinica，2021,54（2）:400-411.
34.Chunsheng Wang ,Shujie Wang , Xiao Ren, Wenjiao Xue, Haijuan He, Yanzhu Zhu, Hongfeng Wang, Gang Wang, Xuehui Cai.Identification of Two Novel Linear Neutralizing Epitopes within the Hexon Protein of Canine Adenovirus Using Monoclonal Antibodies.Vaccines. 2021,9(2):135.
35. Shuyu Wang，Ziyun Ye，Chunsheng Wang*.Research Progress on Bovine Mesenchymal Stem Cells and Related Application[J].Grass-Feeding Livestock，2021,5:1-8.
36. Xiaomeng Wang，Huimin Zhou，Yitong Tong，Shengnan Chen，T.Z.An，Ping Yin，Chunsheng Wang*.Establishment of C2C12 cells with integration of a muscle-specific Cas9-tracking expression vector[J].Acta Laboratorium Animalis Scientia Sinica，2022,30(3)：333-342.
37. Yi Dong，Xinyue Kang，Chunsheng Wang*.Progress on the research of biosynthesis and regulation of mammalian melanin[J].Chinese Bulletin of Life Sciences,2022,34（8）:990-1000.
